Peptides have demonstrated their efficacy as catalysts in asymmetric aldol reactions.But the constraints inherent in chemical synthesis have imposed limitations on the viability of long-chain peptide catalysts.A noticeable dearth of tools has impeded the swift and effective screening of peptide catalysts using biological methods.
To address this, we introduce a straightforward bioprocess for the screening of peptide catalysts for asymmetric aldol theplayfulcollectivees.shop reactions.We synthesized several peptides through this method and obtained a 15-amino acid peptide.This peptide exhibited asymmetric aldol catalytic activity, achieving 77% ee Mug in DMSO solvent and 63% ee with over an 80.
8% yield in DMSO mixed with a pH 9.0 buffer solution.The successful application of our innovative approach not only represents an advancement but also paves the way for currently unexplored research avenues.